Nous recherchons des volontaires pour une étude hydratation : 

  • Femmes d’origine caucasienne
  • Ayant entre 18 et 65 ans
  • Ayant la peau sèche au niveau des avant-bras
  • 1 RDV de 8 heures après pré-sélection (Prévoir un repas froid pour le midi) :
    • >Jeudi 7 Novembre OU
    • Vendredi 8 Novembre
  • Indemnisation: 20 € si vous n’avez pas été incluses
  • Indemnisation: 100 € si vous êtes incluses dans l’étude

Si vous correspondez aux critères, appelez Elisabeth au 01.69.41.42.21 entre 8h et 16h

Nous recherchons des volontaires pour tester un gel douche intime : 

  • Femmes d’origine caucasienne
  • Ayant entre 18 et 45 ans
  • Un examen dermatologique externe des parties intimes sera réalisé un Dermatologue masculin
  • 2 RDVs :
    • Mercredi 13 Novembre (2h si hydratation avant-bras) ET Mercredi 11 Décembre (15min)
    • Indemnisation : 90€
    • Mercredi 13 Novembre (15 min) ET Mercredi 11 Décembre (15min)
    • Indemnisation : 70€

Si vous correspondez aux critères, appelez Elisabeth au 01.69.41.42.21 entre 8h et 16h

Nous recherchons des volontaires pour une étude contour des yeux : 

  • Femmes d’origine caucasienne
  • Ayant entre 35 et 55 ans
  • Présentant des poches ET des rides au niveau du contour des yeux
  • 3 RDVs :
    • Lundi 4 (9h repas du midi fournit) ET Jeudi 7 (20min) ET Mardi 12 Novembre (20 min)
    • OU
    • Mardi 5 (9h repas du midi fournit) ET Vendredi 8 (20min) ET Mercredi 13 Novembre (20 min)
  • Indemnisation : 120€

Si vous correspondez aux critères, appelez Elisabeth au 01.69.41.42.21 entre 8h et 16h

Le Laboratoire BIO-EC vous encourage à lire l’article paru dans le H&PC magazine de juillet /aout (Vol.14) concernant la discussion sur les tests d’efficacité et de sécurité. BIO-EC a eu le privilège d’intégrer la table ronde virtuelle d’experts pour vous présenter son modèle d’explants de peau ex vivo et ses innovations.

Ex vivo and efficacy tests for H&PC Today 2019

Editor’s note: In this issue we present a Panel Discussion on Testing. Experts from testing companies have been invited to participate around a virtual table and discuss about the more interested tests in vitro – considering safety and efficacy tests – ex vivo, silico and in vivo – concerning claims, methods and subjects. Moreover, interviewees have entered into details of the innovations they are working on to answer to customers and consumers’ needs (Tks Publisher, Gayle De Maria).

Living human skin explants is a model of excellence for testing cosmetic products

To evaluate raw materials, active ingredients used in cosmetic products or to test finished products, several biological models are available: in vitro (cell culture, etc.), ex vivo (reconstructed epidermis, living skin) and in vivo (clinical tests on volunteers).

In vitro models are mainly used at the beginning of development to select raw materials or to evaluate their toxicity. Ex vivo models are then used to confirm activity of these cosmetic ingredients, but also to test new formulations or finished products. Clinical tests on volunteers provide evidence of the efficacy of the finished product before marketing. All these models have advantages and disadvantages, but they are complementary for the development of effective cosmetics with proven safety.

Ex vivo model allows to test active ingredients or cosmetic products on human skin at the conditions closest to reality. In addition, it offers a wide variety of analytical techniques: biochemical assays, histological analyses, genomics or physical measurements. Ex vivo model was developed in the 1970s, but only 25 years later, in 1995 this technology has been proposed to test some cosmetic products by the Research Group for Dermatology and Cosmetology (Gredeco). In 1998, BIO-EC Laboratory developed its ex vivo model specifically for the cosmetic industry. For 20 years, ex vivo studies have demonstrated that explants of living human skin are a useful model in understanding the mechanisms of action of active ingredients and evaluation of finished products.

Different types of skin explants can be prepared: whole skin explants (epidermis-dermis-hypodermis), explants of normal skin (epidermis-dermis), explants of adipose tissue (hypodermis) as well as isolated hair from the scalp (figure 1).

Living human skin explants

Figure 1. Different types of explants are available for ex vivo conditions.

Being very close to the skin in vivo, the explants comprising epidermis and dermis allow to test all products intended for topical application (Figure 2). Raw materials can be tested both in a simple formulation applied on the surface of the skin explants or incorporated into the culture medium. Injectable products can be tested as well.

This variety of testable elements represents one of the major advantages of the ex vivo skin explant model compared to in vitro or reconstructed skin models.

BIO-EC exclusive model for cosmetic tests

Figure 2. Explants of living human skin (epidermis + dermis, ± 1cm²) in ex vivo conditions.

Gene expression profiling from RNA extracted from skin explants using RNA microarray technology is of particular interest for cosmetics. Indeed, it is possible to screen potential activities of an active ingredient in an experiment closest to real cutaneous physiology.

As the closest model to real skin, ex vivo explants allow to reproduce various conditions that skin faces in real life, whether it is about natural environment (climate, sun, skin flora), external aggressions (pollution, chemical irritation) or others (wounds, metabolic disorders).

This model is well suited to evaluate the majority of activities of cosmetics – hydration, sunscreen, pigmentation, anti-ageing, slimming or healing activity etc.

For over thirty years human skin explant model has been steadily improving. Thanks to the culture conditions cellular activity and tissue physiology are maintained in a functional state for about 2 weeks. Its complete biological structure cannot be questioned, making it possible to carry out studies in conditions close to reality: a good correlation exists between this model and in vivo studies. Ex vivo model allows to evaluate effects of raw materials as easily as formulations, finished products or medical devices. Explants are useful for several investigation methods such as biochemical, physicochemical, histological, microbiological or genomic analyzes.

Summarizing, ex vivo human skin is a model of choice for evaluating raw materials taking into account its absorption and diffusion into human skin and for validating a product before carrying out an in vivo study.

Venez découvrir nos dernières innovations de tests, notamment sur désordres cutanés, et parler de vos projets d’objectivations, sur le salon COSMETIC 360° les 16 et 17 octobre au Carrousel du Louvre à Paris.

Nous serons présents sur le stand S5 avec notre partenaire, le Laboratoire GENEX, expert des projets cosmétogénomiques (http://www.laboratoire-genex.fr/).

N’hésitez pas à nous contacter pour programmer un rendez-vous avec nous durant le salon !

Le laboratoire BIO-EC a participé à la 25ème édition de l’IFSCC qui s’est déroulée du 30 septembre au 2 octobre à Milan !

Vous pouvez découvrir notre poster : “A new way to investigate the collagen glycation process through its optical properties”.

Nous avons fait une présentation : “Air pollution alters the physicochemical properties of skin surface and affects the physiology of the epidermis in a layer-specific manner”.

N’hésitez pas à nous contacter pour obtenir la version pdf du poster si vous voulez en savoir plus !